il-6 (cat. no. 900 m16)  (PeproTech)

Journal: Scientific Reports

Article Title: Adenosine deaminase modulates metabolic remodeling and orchestrates joint destruction in rheumatoid arthritis

doi: 10.1038/s41598-021-94607-5

Figure Lengend Snippet: Showing ADA Activity and Cytokine levels in Rheumatoid arthritis patients. ( a ) ADA activity in RA patients representing group with Low ADA and High ADA when compared to healthy controls. ( b ) ADA activity of two isoenzymes ADA 1 and ADA 2 (*representing a p value of ≤ 0.05) ( c ) Levels of Cytokines in the serum of RA patients. ( d ) One-way ANOVA analysis of Cytokines and ADA levels in healthy controls, Low ADA and High ADA RA patients. (p-value of ≤ 0.05 is considered significant). All the plots were made using Origin 2020.

Article Snippet: TNFα (Cat. No. CHC1753), TGFβ (Cat. No. CHC1683), IFNγ (Cat. No. CHC1233), and IL-10 (Cat. No. CHC1323) (Invitrogen Cytoset Antibody Pair Kit , California, USA) and IL-6 (Cat. No. 900 M16) and sRANKL (Cat. No. 900 M142) (Peprotech Mini ABTS ELISA Kit, New Jersey, USA) were estimated using manufacturers’ protocols and calculated by the 4-parameter curve-fit method.

Techniques: Activity Assay

Journal: Scientific Reports

Article Title: Adenosine deaminase modulates metabolic remodeling and orchestrates joint destruction in rheumatoid arthritis

doi: 10.1038/s41598-021-94607-5

Figure Lengend Snippet: ADA is involved in joint remodeling in rheumatoid arthritis—in vitro studies to investigate the role of ADA in joint remodeling in the affected joint. ( a ) Increased proliferation of Synoviocytes, ( b ) Decreased cell viability in primary chondrocytes. ( c ) Increased Osteoclast differentiation of macrophages and ( d ) qPCR analysis showing the expression of osteoclast markers after 72 h exposure to ADA. ( e ) Increased cytokine levels in the spent medium after 72 exposure to ADA. ( f ) P2Y 2 , P2Y 4 , and P2Y 6 receptor expression in macrophages after 72 h of ADA exposure. (showing *representing a p value of ≤ 0.05, and **representing a p value of ≤ 0.005). ( g ) Increased ROS in macrophages after ADA exposure (p values of ADA to CTRL on all time points are ≤ 0.005). All the plots were made using Origin 2020.

Article Snippet: TNFα (Cat. No. CHC1753), TGFβ (Cat. No. CHC1683), IFNγ (Cat. No. CHC1233), and IL-10 (Cat. No. CHC1323) (Invitrogen Cytoset Antibody Pair Kit , California, USA) and IL-6 (Cat. No. 900 M16) and sRANKL (Cat. No. 900 M142) (Peprotech Mini ABTS ELISA Kit, New Jersey, USA) were estimated using manufacturers’ protocols and calculated by the 4-parameter curve-fit method.

Techniques: In Vitro, Expressing

Journal: Biomedicines

Article Title: Tauroursodeoxycholic Acid Protects Retinal Pigment Epithelial Cells from Oxidative Injury and Endoplasmic Reticulum Stress In Vitro

doi: 10.3390/biomedicines8090367

Figure Lengend Snippet: TUDCA suppressed H 2 O 2 -induced inflammation. ( A ) IL-1β, IL-6, and TNF-α mRNA levels in control, H 2 O 2 , and H 2 O 2 -plus-TUDCA-treated cells were measured by qRT-PCR. ( B ) Protein concentrations of IL-1β, IL-6, and TNF-α in control, H 2 O 2 , and H 2 O 2 -plus-TUDCA-treated cells were detected by ELISA. Data were shown as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001, 750 µM H 2 O 2 vs. untreated (UT) control; ## p < 0.01, ### p < 0.001, #### p < 0.0001, 750 µM H 2 O 2 plus TUDCA vs. 750 µM H 2 O 2 .

Article Snippet: Human Interleukin 1 beta (IL-1β) Mini ABTS ELISA Development Kit (Cat. 900-M95), IL-6 Mini ABTS ELISA Development Kit (Cat. 900-M16), and tumor necrosis factor alpha (TNF-α) Mini ABTS ELISA Development Kit (Cat. 900-M25), all purchased from PeproTech, London, UK, were used to measure, respectively, IL-1β, IL-6, and TNF-α, based on the manufacturer’s protocols.

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay